Fig. 5: mRNA orientation within germ granules.

a STED images of smFISH of wild-type embryos with nos 5′end (5′-nos, magenta) and 3′end (3′-nos, green) probes. b, c STED images of immuno-smFISH with anti-Osk antibody (magenta) as a marker of germ granules and nos 5′end (b) or 3′end (c) probes (green). d Percentage of nos 5′end and 3′end foci localized in the core (black), in and at the surface of the shell (grey), and at the immediate periphery (white) of germ granules from images as in (b, c). e Radar plot of the relative localization of nos 5′end (magenta dots) and 3′end (green dots) within Osk immunostaining (blue) in wild-type embryos from images as in (b, c). The granule shell is in blue. f STED images of smFISH of wild-type embryos with CycB 5′end (5′-CycB, magenta) and 3′end (3′-CycB, green) probes. g, h, STED images of immuno-smFISH with anti-Osk antibody (magenta) as a marker of germ granules and CycB 5′end (g) or 3′end (h) probes (green). i Percentage of CycB 5′end and 3′end foci localized in the core (black), in and at the surface of the shell (grey), and at the immediate periphery (white) of germ granules from images as in (g, h). j Radar plot of the relative localization of CycB 5′end (magenta dots) and 3′end (green dots) within Osk immunostaining (blue) in wild-type embryos from images as in (g, h). The granule shell is in blue. Scale bars: 1 µm. Source data are provided as a Source Data file.