Fig. 2: Scar transforms decidual fibroblasts into NF-κB mediated inflammatory state.

A Representative immunohistochemistry images of maternal-fetal interface (MFI) tissue sections from PAS patients showing EVTs present in decidual regions proximal, and distal to collagenous acellular scar regions; Quantification of EVT density in either region in right panel; n = 5 and 3 for regions proximal and distal to scar respectively; EVTs and dESFs are marked with HLA-G (red; arrow heads) and Vimentin (green), respectively. p = 0.005. B KEGG pathway enrichment analysis showing signaling pathways differentially enriched in dESFs on Scar and Physio; C Gene ontologies related to inflammation enriched in dESFs on Scar and Physio. D Representative immunofluorescence images showing RelA (p65) location in dESFs on Physio and Scar; Quantification showing percentage of dESF with nuclear RelA in lower panel. n = 4 and 5 fields of view for Physio and Scar, respectively. p = 5 × 10⁻⁸. E Immunoblot showing abundance of phosphorylated RelA (p-RelA) in dESFs on Physio and Scar. Experiments are repeated twice with similar results. F ANSIA based analysis of stromal invasion of primary EVTs into dESF compartment with scrambled, or gene silenced for NFKB1; n = 10 and 13 locations for scrambled and NFKB1^KD, respectively. p = 0.005. G Representative immunohistochemistry images of MFI tissue sections from PAS patient showing RelA intracellular localization in decidual regions proximal, and distal to scar; H Quantification of percentage of decidual fibroblasts with nuclear RelA; n = 3 and 6 locations for distal and proximal, respectively. p = 0.0005. I Pearson correlation test shows Pearson coefficient (r) of EVT number per field of view, and ratio of decidual fibroblasts with nuclear and cytoplasmic RelA and total decidual fibroblasts in PAS MFI tissue sections; a two-tailed p-value for Pearson’s r is calculated; n = 8 field of views. Data in figures A, D, F, H are showing as mean ± s.d.; statistical significance is determined by unpaired two-tailed t-test (**p < 0.01, ***p < 0.001). Source data are provided as a Source Data file.