Fig. 3: Analysis of mAb binding by HCI to a curated collection of CC92 A. baumannii strains isolated in Vietnam between 2003 and 2018. | Nature Communications

Fig. 3: Analysis of mAb binding by HCI to a curated collection of CC92 A. baumannii strains isolated in Vietnam between 2003 and 2018.

From: Exploiting human immune repertoire transgenic mice for protective monoclonal antibodies against antimicrobial resistant Acinetobacter baumannii

Fig. 3

Individual bacteria were stained and segmented using DAPI which stains intracellular bacterial DNA (a), antibody binding was measured by the indirect signal from binding human IgG1 mAb using an Alexa Fluor 647 labelled secondary anti-human IgG1 (b) overlay of both signals (c). d Bacterial surface mAb binding at 1 μg/ml across the entire phylogenetically characterised CC92 collection. Binding was defined as positive where the Alexa Fluor 647 mean intensity per well was >500 relative fluorescent units (RFU).

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