Fig. 6: Ability of mAbs to protect mice against neonatal sepsis causing strains of A. baumannii.

a Temporal phylogenetic characterisation of carbapenem-resistant A. baumannii strains isolates from Vietnamese healthcare settings between 2003 and 2018. Phylogeny was built using whole genome sequence data from the calling of single nucleotide polymorphisms from core genes using RAxML (Randomised Axelerated Maximum Likelihood). b Adult C57BL6 mice were intravenously dosed with 10, 5 or 1 mg/kg of 1416 and 10 mg/kg isotype control mAb on day -1 and day 0, challenged on day 0 with 5 × 10⁷ CFU A. baumannii isolate CM10420. Bacterial loads at 24 h in lungs were enumerated as CFU/gram of lung tissue (CFU/g) to evaluate protection. Statistical analysis was performed using a Mann–Whitney test with p shown as a numerical value when p ≤ 0.05. The data presented are from a single study (n = 5; n defined as an individual mouse). c Sunspot plot showing antibody sequence clustering for different bacterial antigen targets. Colour of symbol in index indicates target bound by individual mAb. Symbol shape in index indicates individual mouse from which corresponding VH/VL sequence pair was derived (indicated by nominative code identifier in the index). The mAb 1416 is indicated in a large cluster (indicated by enclosed region with blue Asterix).