Fig. 2: T-ALL hyperinteracting hubs are markedly transcribed and organize expression of genes encoding transcription factors and cofactors.
From: Enhancer-promoter hubs organize transcriptional networks promoting oncogenesis and drug resistance
a Enhancer-promoter hubs detected from T-ALL DND41 Hi-C data are plotted in ascending order of their total interactivity. The purple region marks hyperinteracting hubs, defined as hubs above the elbow of the total interactivity ranking curve. b Plot of Hi-C interaction count vs. enhancer/promoter element count in each T-ALL DND41 hub with hyperinteracting hubs marked in purple. c Plot of Hi-C interaction count vs. ratio of Hi-C interaction to enhancer/promoter counts in each T-ALL DND41 hub with hyperinteracting hubs marked in purple. d Median gene expression of T-ALL DND41 Hi-C hubs vs. 5000 sets of matched, randomly selected regions (empirical permutation p-value). e Median gene expression of T-ALL DND41 Hi-C hyperinteracting hubs vs. 10,000 sets of matched, randomly selected regions (empirical permutation p-value). f Box-and-whisker plots comparing transcription levels of T-ALL DND41 Hi-C regular (n = 1219) and hyperinteracting (n = 158) hubs. Box-and-whisker plots: center line, median; box limits, upper (75th) and lower (25th) percentiles; whiskers, 1.53 interquartile range. P-value: two-tailed Wilcoxon rank sum test. g Molecular function GO enrichment analysis of DND41 SMC1 HiChIP hyperinteracting hubs. P-value: Fisher’s exact test. h Overlap of T-ALL DND41 hubs identified from Hi-C and SMC1 HiChIP data. Percentages and counts of overlapping and unique hubs are shown separately for Hi-C and SMC1 HiChIP. i Overlap of T-ALL DND41 hyperinteracting hubs identified from Hi-C and SMC1 HiChIP data. Percentages and counts of overlapping and unique hyperinteracting hubs are shown separately for Hi-C and SMC1 HiChIP.
