Fig. 5: Mode of action of DBSA compared with those of reported ABA receptor agonists and antagonists. | Nature Communications

Fig. 5: Mode of action of DBSA compared with those of reported ABA receptor agonists and antagonists.

From: Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination

Fig. 5

a Binding mode of different ligands with PYLs. PYL1-DBSA (PDB code 9J6I), PYR1-AS6 (PDB code 3WG8), PYL10-antabactin (PDB code 7MLD), PYR1-pyrabactin (PDB code 5UR4), PYL2-quinabactin (PDB code 4LA7) and PYL10-3CB (analog of opabactin, PDB code 6NWC). PYR1-pyrabactin, PYL2-quribaction, PYL10−3CB, PYL10-antabactin and PYL5-AS6 are in latch-closed and gate-closed conformations, whereas PYL1-DBSA is in latch-open and gate-open conformation. For the latch-closed and gate-closed agonist binding conformations, a conserved Trp lock of PP2Cs were insert into the PYL pockets. b Mode of action of ABA receptor agonists, ABA-mimic receptor antagonists AS6, antabactin and DBSA. ABA receptor agonists cause gate-closed conformations and PYL dimer dissociation, which inhibits PP2Cs. ABA-mimic receptor antagonists AS6 and antabactin also cause gate-closed conformations and PYL dimer dissociation, but AS6 obstructs the interaction between PP2C and PYLs by occupying the 3’ tunnel, and antabactin blocks the conserved Trp lock of HAB1 to PYL. DBSA stabilizes the PYL dimer, which results in PP2C activation.

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