Fig. 3: Wastewater bacterial phylogenetics and diversity.

a The average relative abundance (RA) of all bacterial families represented in the metatranscriptomic data. Outer rings correspond to mean abundance within a family across the different location types extending from the WWTP wastewater treatment plant (outermost ring) to SCHOOL primary/secondary school, UC university campus, DORM dormitory, and HOSP hospital, (innermost ring). The color of the family name corresponds to its phylum. b The top panel is the beta diversity (Bray Curtis distance) between all samples within a given site. Each point in this panel represents a comparison between two different samples taken from the same location (e.g., two random samples from a given hospital). The line underneath the top panel corresponds to the average beta diversity at each associated site. The dots in the Population Size sub-panel correspond to the approximate population served by the site from which samples are being compared between. The colored blocks on the bottom are vertically aligned with the population size points and the beta diversity dotplots, and colors correspond to the different sampling location types indicated in the legend. Boxplots represent the median (center line), the 25th (lower bound of the box), and 75th (upper bound of the box) percentiles. The whiskers extend to the smallest and largest values within 1.5 times the interquartile range (IQR) from the lower and upper quartiles, respectively. c The intersections between different location types, with the bars indicating the intersection size and the black dots indicating the sites underneath being compared. These bars are vertically aligned with the middle and top panels, which show the relative abundance and prevalence, respectively, of all bacterial species represented by each bar. d The log10 RA of bacteria potentially associated with any location type in our Microbial Association Study (MAS). Bacteria occurring in at least three samples and with a BY-adjusted p-value of less than 0.1 are plotted. Orange names indicate gut commensals. Source data are provided as a Source Data file.