Fig. 7: Poly-purine and -pyrimidine rich 3’-UTRs in bloodstream up-translated mRNAs.

a The motif shown was enriched in 3’-UTRs that displayed >5-fold upregulated translation⁵ in bloodstream-form cells relative to control UTRs ( < 10% difference between life-cycle stages; n = 1450). b The violin plots on the left show motif density for 3’-UTRs of >250 b that displayed >4-fold upregulated mRNA abundance in bloodstream-form cells (BF-up; n = 139), or insect-stage, procyclic form cells (PF-up; n = 73), relative to control UTRs ( < 10% difference between life-cycle stages; n = 999). The violin plots on the right show motif density for 3’-UTRs of >250 b that displayed >4-fold upregulated translation in bloodstream-form cells (BF-up; n = 278), or insect-stage, procyclic form cells (PF-up; n = 81), relative to control UTRs ( < 10% difference between life-cycle stages; n = 818). Data are shown for the A-rich poly-purine motif in Fig. 4b, and for the U-rich poly-pyrimidine motif in Fig. 7a, (FIMO settings ‘p < 0.01’). Open circles indicate median values while t-tests were one-sided. c The plots show density of A-rich motifs and U-rich motifs (FIMO settings ‘p < 0.01’), and 3’-UTR length and also published ratios of translation efficiency⁵ for the bloodstream form up-translated and procyclic form up-translated sets of transcripts described in (b). The plot on the right shows data for those six exemplar 3’-UTRs detailed in (d, e), four from the BF-up set and two from the PF-up set. d The maps show UTR-seq read-density for four exemplar 3’-UTRs associated with bloodstream form up-translated genes and with positive regulatory hit fragments in the screen. Tracks showing nucleobase density are included. Other details as for Fig. 3c. e The maps show UTR-seq read-density for two exemplar 3’-UTRs associated with insect-stage up-translated genes and with negative regulatory hit fragments in the screen. Tracks showing nucleobase density are included; other details as for Fig. 3d.