Fig. 1: Binding of antibodies with heavy chain variable regions derived from deep sequencing to TREM2.

Binding of antibodies to TREM2 peptides 129–148 and 149-168 and mouse inhibin 30-mer control in ELISA is shown in a heatmap with optical density scale on the right. Readings below 0.35 are considered negative. The V_H regions of antibodies are shown on the left by their corresponding hybridoma or V_H clone name. V_H clone names refer to rat number, CDR H3 length and unique number. The light chains of the antibodies are shown on the top using the corresponding hybridoma clone name for the light chain or the V_L germline segment of three other anti-TREM2 antibodies. Hybridoma-derived heavy and light chain variable regions are highlighted in salmon. The epitope region of the antibodies from which light chains are derived are indicated. Dashes indicate lack of expression. Regions shown in gray are antibodies with heavy and light chain combinations that were not tested. Control (Ctrl.) antibodies 1 and 2 bind TREM2 129–148 and 149–168, respectively. Ab 4D5 is an anti-human epidermal growth factor receptor 2 antibody. The relative number of read counts with the V_H10-5 germline segment and CDR H3 sequences from the hybridoma or selected clones in the deep sequencing datasets of rats R18, R19 and R20 are shown on the right in blue shading as indicated by the color scale and percentage shown in boxes. Zero values indicate less than 0.5% and blank values indicate no reads found. The maximum CDR H3 sequence identities (excluding the intra-clonal 3.10A7/3.22B9 similarity) among TREM2-binding clones of the same CDR H3 length or different lengths with one or two contiguous gaps are indicated on the right. OD, A₄₅₀ ELISA optical density. Source data are provided as a Source Data file.