Fig. 3: Network analysis design and findings.
Network analysis approach to evaluate the impact of undersown diversity on microbial associations within the rhizosphere of barley across the diversity gradient (a). Bacterial networks showing edges in blue if representing positive associations or in red if representing negative associations and the colors of the nodes represent the different bacterial phylum within each plant diversity treatment from top to down: barley monoculture, barley + 1, barley + 4 and barley + 8, respectively (b). The Ratio of positive to negative network parameter of degree centrality which captures network connectivity at each undersown diversity treatment (c). Significant differences between treatments are indicated by different letters (linear mixed effects models with block as the random effect using the nlme package, and ANOVA type III to correct for unbalanced design, P = 5 × 10−7, df = 163, n = 167). In the boxplots, whiskers denote the minimum value or 1.5× interquartile range (whichever is more extreme), and box denotes interquartile range. The horizontal line denotes the median. Biological replicates: n = 24, 95, 24 and 24 for barley monoculture, barley +1, barley + 4 and barley + 8, respectively. Source data are provided as a Source Data file for Fig. 3c.
