Fig. 5: Morphology and growth fitness of the nucleosome-forming E. coli.
From: Creating a bacterium that forms eukaryotic nucleosome core particles
a Representative images for visualization of the nucleosome-forming (Ec-r-pXen) and control (Ec-r-pET29a) strains from an optical microscope (“Methods”). DAPI was used to stain E. coli DNA. BF, bright field. Bar length = 10 µm. E. coli cells were grown at 37 °C and treated with 1 µM IPTG for long-term growth. The figure is representative of two independent experiments. b Cell length distribution of the nucleosome-forming (Ec-r-pXen) and control (Ec-r-pET29a) strains. The violin plots show the summary statistics (the median, first and third quartiles, 1.5 × interquartile range) and kernel distribution of cell length. Plots are generated from the measurement of about 250 cells across 10 independent areas for each strain. A two-sided Mann-Whitney Rank Sum Test was performed to obtain the p-value. c Optical density measurement of cell growth (mean ± SEM) of the nucleosome-forming (Ec-r-pXen) and control (Ec-r-pET29a) strains at 37 °C in LB media with 1 µM IPTG. Data represent twenty-four biological replicates (n = 24). d Growth competition assay of the nucleosome-forming (Ec-r-pXen) and control (Ec-pET29a-ampR) strains at 37 °C in LB media with 1 µM IPTG (“Methods”). Data represent four biological replicates (n = 4). Source data are provided as a Source Data file.
