Fig. 1: Screening for TadA orthologs with cytosine deaminase activity in mammalian cells. | Nature Communications

Fig. 1: Screening for TadA orthologs with cytosine deaminase activity in mammalian cells.

From: Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation

Fig. 1

a The experimental workflow for detecting base editing activity with fluorescence reporter system in HEK293T cells. b The Phylogenetic Tree of TadA orthologs. c Screening for TadA orthologs using fluorescence reporter system. Data are presented as means ± S.D. Values and error bars represent mean and S.D., n = 3 independent biological replicates. All of the above base editors do not contain UGI. PAM stands for protospacer adjacent motif. NC represents negative control. Source data are provided as a Source Data file.

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