Fig. 2: Engineering of AjTadA to improve base editing efficiency in mammalian cells. | Nature Communications

Fig. 2: Engineering of AjTadA to improve base editing efficiency in mammalian cells.

From: Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation

Fig. 2

a The experimental workflow for engineering of AjTadA. b Substitutions of non-positively charged amino acids of AjTadA at the AHC sites. Each dot represents activity for a single variant. c Substitutions of 25E and 46 P of AjTadA.v1 with seven amino acid residues. Values represent mean, n = 2 independent biological replicates. d Combination of mutations in 25E and 46 P of AjTadA.v1. Values and error bars represent means ± S.D., n = 3 independent biological replicates. e Combination of single amino acid mutation with AjTadA.v2 at three endogenous loci. Values and error bars represent means ± S.D., n = 3 independent biological replicates. All of the above base editors do not contain UGI. Source data are provided as a Source Data file.

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