Fig. 5: In vitro promotion of the cGAS-STING pathway. | Nature Communications

Fig. 5: In vitro promotion of the cGAS-STING pathway.

From: Biodegradable oxygen-evolving metalloantibiotics for spatiotemporal sono-metalloimmunotherapy against orthopaedic biofilm infections

Fig. 5

a Schematic illustration of the promotion of the cGAS-STING pathway. b, c Western blot of the activation of the cGAS-STING pathway in APCs (RAW264.7 and DC2.4 cells) after different treatments. Actin = 42 kDa, IRF3 and p-IRF3 = 46 kDa, TBK and p-TBK = 84 kDa. Relative gene expression of the cGAS-STING axis in RAW264.7 (d) and DC2.4 (e) cells after different treatments by qPCR analysis. f ELISA detection of Ifn-I, Tnfα, and Il6 in RAW264.7 and DC2.4 cells after different treatments. n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. g The efficacy of promoting DC maturation in different groups evaluated by flow cytometry in vitro with quantitative analysis (h). n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. i CLSM images showing STING activation in DCs with quantitative analysis (j), where blue represents cell nuclei and red represents STING. Scale bar, 50 μm. n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. Source data are provided as a Source Data file.

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