Fig. 8: In vitro pro-tissue repair evaluation.

a Schematic of posttreatment with SMIT therapy. Sustained oxygenation from MnP without US accelerated wound healing. b Flow cytometry of CD80 and CD206 expression on macrophage after different treatments. c M2-phenotype macrophage percentage. n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. d Relative gene expression of RAW264.7 cells after different treatments by qPCR analysis. e ELISA detection of Il10, Vegf, Il6, and Tnfα in RAW264.7 cells after different treatments. n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. f Scratch assay of EA.hy926 cells cultured in macrophage-conditioned media for 0 and 24 h with quantitative analysis of the migration ratio (g). Scale bar, 200 μm. n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. h Transwell migration assay of EA.hy926 cells in different groups with quantitative analysis of the number of migrated cells (i). Scale bar, 200 μm. n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. j Vascular tube formation of EA.hy926 cells cultured in various macrophage-conditioned media on Matrigel for 6 h with quantitative analysis of the number of circles (k). Scale bar, 200 μm. n = 3 independent experiments; means ± SDs. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using two-tailed Student’s t tests. Source data are provided as a Source Data file.