Fig. 5: MS023 treatment results in a stalled cell cycle and eventual cell death. | Nature Communications

Fig. 5: MS023 treatment results in a stalled cell cycle and eventual cell death.

From: PRMT1 inhibition perturbs RNA metabolism and induces DNA damage in clear cell renal cell carcinoma

Fig. 5

a Western blot analysis of mitotic checkpoint protein BUB1B and centromere proteins CENPA and CENPI in RCC243 cells treated with 0 μM, 0.1 μM, 5 μM, and 10 μM of MS023 for 3 days. b Western blot analysis of phospho-histone H3 in RCC243 cells treated with 0.1 μg/mL Nocodazole (beta-tubulin disruptor and mitotic staller), 100 μM etoposide (DNA damaging agent) and 5 μM MS023 for 3, 5 and 7 days. c Flow cytometric histograms showing cell cycle progression of RCC243 cells in response to 5 μM MS023 inhibition over 9 days. Source data are provided as a Source Data file.

Back to article page