Fig. 2: The MFSD7C expression level was downregulated in alveolar macrophages and endothelial cells of hemolytic lung.

a Relative MFSD7C protein levels in various tissues of 8 weeks old HbAA-Townes mice. n = 3. b–d 12-month old HbAA- and HbSS-Townes mice were analyzed. b Expression levels of MFSD7C and HMOX1 in lung tissues were analyzed using western blot. Three biological replicates were performed for each sample. c Changes in the mRNA expression of MFSD7C of lung tissues from hemolytic mouse model were evaluated by RT-qPCR. n = 5. d IHC staining of MFSD7C in lung samples. e Relative MFSD7C mRNA level in AMs, epithelial cells, and endothelial cells isolated from lung tissues. n = 5. f Representative immunofluorescence images of lung tissues in HbAA mice (MFSD7C in green, CD31 and F4/80 in red, and nuclei in blue, photomicrograph bar = 5 µm). Data are presented as mean + standard deviation (SD). *p < 0.05; **p < 0.01; ***p < 0.001.