Fig. 5: MFSD7C deficiency increased lipid flux by inducing CD36 and FABP4 expression.

a Schematic diagram for fatty acid transportation and oxidation. b Heatmaps of relative gene expression in MFSD7C-KO HuLEC-5A cells versus control. c RT-qPCR analysis of CD36, FASN, FABP4, CPT1A, SLC27A3, SLC27A4, SLC27A5 and ACOX1 relative mRNA levels in AMs from 7C^flox/flox and 7C^−/− mice. n = 3. d Representative western blot of CD36 and FABP4 expression in AMs. Three biological replicates were performed for each sample. e Western blot analysis of CD36 and FABP4 knockout efficiency in MFSD7C-WT and KO HuLEC-5A cells. Three biological replicates were performed for each sample. Uptake of long chain FAs (Bodipy C16) in CD36 KO (f) or FKBP4 KO (g) HuLEC-5A cells was determined by FACS analysis. h–i Lipid ROS production in HuLEC-5A cells were determined by flow cytometry using BODIPY C11. Seahorse analysis of cellular oxygen consumption rate (OCR) (j), basal respiration (k), maximum respiration (l), and proton leak (m) in CD36 KO HuLEC-5A cells. n = 3. Data are presented as mean + standard deviation (SD). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.