Fig. 2: The Comamonas DA1877 diet changes germ cell number and dynamics.

a Oocyte number for indicated genotypes. Dots represent individual oocytes. b Mitotic index for indicated genotypes. Dots represent individual animals. c Fluorescence images showing the pachytene-to-diplotene (P-D) transition (yellow dashed lines) for indicated genotypes; germ cell nuclei are marked with H2B::GFP. Gonads are outlined by white dashed lines. Scale bar: 10 µm. d Number of cells inside an area starting from the P-D and ending 15 cell-rows distally of indicated genotypes; P-D was determined based on the nuclear marker shown in c; in let-60(gf) animals the loop region was used as the starting point. e Length of 15-cell rows for indicated genotypes. f Distance from the P-D to the loop region for indicated genotypes. d–f dots represent individual animals. g Speed of germ cell progression through the pachytene region for indicated genotypes; for individual tracking see Supplementary Fig. 2. Dots represent the speed of individual cells. h Ovulation time for indicated genotypes. Dots represent individual oocytes. Bars represent mean ± SD. a–h Animals were fed with E. coli OP50 (gray) or Comamonas DA1877 (blue). Number of animals in brackets. Boxplots show 25-75 percentiles and median; whiskers represent min and max values. ****P < 0.0001 using two-way ANOVA followed by uncorrected Fisher’s LSD multiple comparison test in a, one-way ANOVA followed by Dunnett’s multiple comparison tests in b, d–h. Source data are provided as a Source Data file.