Fig. 3: Differential spatial distributions between tumor draining lymph nodes (TDLN) with cold and hot tumors. | Nature Communications

Fig. 3: Differential spatial distributions between tumor draining lymph nodes (TDLN) with cold and hot tumors.

From: Tumor draining lymph nodes connected to cold triple-negative breast cancers are characterized by Th2-associated microenvironment

Fig. 3

A, B Representative regions from mIF stained images showing the spatial distributions of mature dendritic cell (DC), T helper 1 (Th1), and T helper 2 (Th2) cells in TDLNCold (A) and TDLNHot (B). Whole slide images for these two samples are shown in Fig S4A, B. C Higher ratios of Th2 to Th1 cell numbers (p = 6.0 × 10−4) in TDLNCold (n = 7) compared to TDLNHot (n = 7). D Higher Th2 percentages to total CD4+ T cells (p = 6.0 × 10−4) in TDLNCold (n = 7) compared to TDLNHot (n = 7). E Similar cluster percentages of mature-DC-Th1 cluster (relative to total cluster number, p = 0.53) between TDLNCold (n = 7) and TDLNHot (n = 7). F Higher cluster percentages of mature-DC-Th2 cluster (relative to total cluster number, p = 0.05) in TDLNCold (n = 7) compared to TDLNHot (n = 6) for mature-DC-Th1 clusters. G The averages of cluster percentages across Th1/2 cell types and TDLNCold/Hot. Data in (C–F) were presented as mean values ± the standard errors of the mean. All the outliers were identified by ROUT method with Q = 1%. The statistical significances based on the two-sided Mann–Whitney test was shown in (C–F). Calculated p values are displayed as “ns”, p > 0.05; *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001. Source data including the outliers are provided as a Source Data file.

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