Fig. 6: In vivo toxin neutralization. | Nature Communications

Fig. 6: In vivo toxin neutralization.

From: De novo design of mini-protein binders broadly neutralizing Clostridioides difficile toxin B variants

Fig. 6

a Fc_H3 and Fc_5N2 refers to H3 and 5N2 fused to the IgG1-Fc fragment, respectively. Fc_H3_5N2 denotes the fusion of H3 and 5N2 to each arm of the bispecific IgG1-Fc. We used saline as the control. Mouse colonic tissues harvested after colon-loop ligation assays were evaluated for the histopathology induced by TcdB1 (b), TcdB2 (c), TcdB3 (d), and TcdB4 (e), both with and without TcdB binders, through H&E staining. Representative images are depicted, with the scale bar indicating a length of 100 μm. The overall histological scores were determined by assessing edema, inflammatory cell infiltration, epithelial disruption, and crypt damage. Six mice were analyzed, and error bars indicate the mean ± SEM. One-way ANOVA using the Tukey test for multiple comparisons. Source data are provided as a Source Data file.

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