Fig. 6: The synovium shares properties with adipose stromal cell populations. | Nature Communications

Fig. 6: The synovium shares properties with adipose stromal cell populations.

From: Adipocyte associated glucocorticoid signaling regulates normal fibroblast function which is lost in inflammatory arthritis

Fig. 6

A UMAP of PDGFRα+ stromal cells from human visceral and subcutaneous depots (VAT and SAT). B Bulk RNA sequencing defined cortisol, FCM, FCM + GCR ant, and TGF-β1 scores applied to adipose clusters. Fibroblasts from each donor were extracted and reads collapsed over donor and cluster to perform a pseudobulk analysis, meaning each data point represents the activation score of all of a donor’s fibroblasts, separated by cluster. A Kruskal–Wallis test with Dunn’s multiple comparison post hoc test was used to calculate significance. n = 28 biological samples. C Symphony UMAP with adipose stromal cells as a reference, quantification of mapping on right. Healthy n = 10, OA n = 9, RA n = 25, REM n = 3 biological samples. P values were calculated using an ordinary one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. B, C Statistical comparisons: all groups were compared to each other. All data are presented as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.

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