Fig. 6: Conservation of the interaction between Marchantia and the TMV silencing suppressor p126. | Nature Communications

Fig. 6: Conservation of the interaction between Marchantia and the TMV silencing suppressor p126.

From: Conservation of molecular responses upon viral infection in the non-vascular plant Marchantia polymorpha

Fig. 6

A p126-GFP shows accumulation in the cytoplasm of Marchantia cells in cross-sections of thalli from 4 weeks old plants (right) compared to thalli from untransformed plants of the same age plants (left). The pictures show the merge between green (GFP) and red (autofluorescence) channels. P stands for Photosynthetic region, S for storage region and E stands for lower epidermis. Merge was performed using Fiji Scale bars are 100 µMs. B Overexpression of p126 inhibits growth (area) when compared to Tak-1 control plants. Analysis was done in 4 weeks old plants in 3 biological replicates (n = 12). Statistical significance was assessed using a two-sided Wilcoxon-test with Holm p adjustment. Length of the whiskers: 1.5 × IQR. Central line: median. Box width: IQR (i.e., Q3–Q1). C MpNAC7 interacts with the helicase domain of p126 in yeast. D MpNAC7 and ATAF2 interact both with the helicase and the full length p126 in planta. E Transient expression of MpNAC7 in Nicotiana plants abrogates local and systemic TMV infection. TMV presence is shown as purple.

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