Fig. 4: HPLC data for determining the absolute configuration of the amination product. | Nature Communications

Fig. 4: HPLC data for determining the absolute configuration of the amination product.

From: Direct asymmetric synthesis of β-branched aromatic α-amino acids using engineered phenylalanine ammonia lyases

Fig. 4

a In vitro deamination assays with β-MePhe of different absolute configurations using the PcPAL-L256V-I460V mutant. Reaction conditions: 100 µg purified enzyme, 1 mM various β-MePhe, total 500 µL in PBS buffer (pH 8.0), 30 °C, 450 rpm for 24 h. i) (2S, 3S)-β-MePhe; ii) deamination reaction of (2S, 3S)-β-MePhe; iii) (2 R, 3R)-β-MePhe; iv) deamination reaction of (2R, 3R)-β-MePhe; v) (2S, 3R)-β-MePhe; vi) deamination reaction of (2S, 3R)-β-MePhe; vii) β-MeCA standard. b Derivatization results with β-MePhe of different configurations and the in vitro reaction product formed by using Marfey’s reagent. viii) standard mixture of β-MePhe derivatized with Marfey’s reagent; ix) (2S, 3S)-β-MePhe derivatized with Marfey’s reagent; x) (2S, 3R)-β-MePhe derivatized with Marfey’s reagent; xi) derivation of the in vitro amination reaction product produced by the PcPAL-L256V-I460V mutant; xii) (2R, 3R)-β-MePhe derivatized with Marfey’s reagent; xiii) blank containing solely Marfey’s reagent and enzyme. β-MePhe β-methyl phenylalanine, β-MeCA β-methyl cinnamic acid.

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