Fig. 4: Thymic immunopeptidome analysis reveals a broad MHC-II epitope repertoire containing a minor component of islet-derived epitopes. | Nature Communications

Fig. 4: Thymic immunopeptidome analysis reveals a broad MHC-II epitope repertoire containing a minor component of islet-derived epitopes.

From: Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire

Fig. 4

a Schematic for three independent analyses of the thymic MHC-II peptidome, created in BioRender. Wan, X. (2024) BioRender.com/s20b261. b Gibbs cluster analysis of all the identified thymic MHC-II peptides showing an I-Ag7-binding motif. c Tissue localization analysis of the source proteins of the identified thymic MHC-II peptides. d Illustration of a fraction of PTAs that were mapped to different peripheral tissues (y-axis), which also corresponded to genes known to be regulated by Aire, Fezf2, and Chd4 (x-axis). e Cellular component analysis of the source proteins of the identified peptides in the thymic MHC-II peptidome. The pie chart depicts the protein counts of indicated cellular components. f A mirror plot depicts a complete match of the identified B:9–23 sequence (with cysteine oxidized to cysteic acid) via antibody capture in the thymic MHC-II peptidome (upper) with its synthetic standard (lower).

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