Fig. 7: E21-specific T cells derived from NOD.Ins2−/− mice exhibit a nontolerant phenotype. | Nature Communications

Fig. 7: E21-specific T cells derived from NOD.Ins2−/− mice exhibit a nontolerant phenotype.

From: Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire

Fig. 7

a Flow cytometry analysis of FOXP3+ Tregs in E21:I-Ag7 tetramer-binding CD4+ T cells in the thymus of 6-week-old female NOD and NOD.Ins2−/− mice. Data (mean ± s.e.m) summarize results from three independent experiments; each symbol represents results from an individual mouse (n = 6). **P = 0.0022; Mann–Whitney test, two-tailed. b Flow cytometry analysis of FOXP3+ Tregs in E21:I-Ag7 and G20:I-Ag7 tetramer-binding CD4+ T cells in SLOs of 6-week-old female NOD and NOD.Ins2−/− mice. Data (mean ± s.e.m) summarize results from three independent experiments; each symbol represents results from an individual mouse (n = 7 for NOD E21 group, n = 8 for NOD.Ins2−/− E21 group, n = 6 for NOD G20 group, n = 7 for NOD.Ins2−/− group). ns, not significant; ****P < 0.0001; Mann–Whitney test, two-tailed. c ELISPOT assay for assessing T cell responses during antigen recall with intact insulin or Syn-E21 in NOD or NOD.Ins2−/− mice post immunization with the B:9–23 peptide. Data (mean ± s.e.m) summarize results from three independent experiments; each symbol represents results from an individual mouse (n = 5). **P = 0.0079; Mann–Whitney test, two-tailed. The schematic figure is created in BioRender. Wan, X. (2024) BioRender.com/h20l924.

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