Fig. 2: Cryo-EM Structure of SpoIVFB:Pro-σK Complexes.
From: Substrate engagement by the intramembrane metalloprotease SpoIVFB
A Three rotated views of the tetrameric cryo-EM structure of WT SpoIVFB:Pro-σK. SpoIVFB is colored blue, Pro-σK is colored orange, LMNG detergent is colored magenta, a lipid-like density at the interface between monomers is colored green, and the detergent micelle is shown in light transparent gray. B Selected 2D averages of the tetrameric WT SpoIVFB:Pro-σK complex showing four copies of WT SpoIVFB:Pro-σK arranged with overall 2-fold symmetry. C Three rotated views of the dimeric cryo-EM structure of E44Q SpoIVFB:Pro-σK. Coloring is the same as in (A). D Selected 2D averages of the dimeric E44Q SpoIVFB:Pro-σK complex showing two copies of E44Q SpoIVFB:Pro-σK arranged with no symmetry.
