Fig. 1: Label-free quantitative proteomics analysis of RPL5-FLAG co-purified proteins.

Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) analysis of trypsin digested proteins retained on flag-coated beads issued from U2OS control cells (LEFT quadrant) or cell expressing RPL5-FLAG (RIGHT quadrant). Three independent experimental replicates (n = 3) were performed. Volcano plots showing proteins significantly enriched for control cells (red) versus cells expressing RPL5-FLAG (a) in untreated cells (b) in cells treated with actinomycin D (ACTD, 10 ng/ml) for 24 h. An unpaired two-tailed Student’s t test with equal variance was used. Enrichment significance thresholds are represented by an absolute log2-transformed fold-change (FC) greater than 1 and a -log10-transformed (p-value) greater than 1.3. The iBAQ (intensity-Based Absolute Quantification) values are represented by the diameter of each dot for the proteins that are significantly enriched in order to rank their absolute abundances⁷³. Source data are provided as a Source Data file.