Fig. 6: SURF2 overexpression impedes p53 activation and cell cycle arrest following nucleolar stress.
From: SURF2 is a MDM2 antagonist in triggering the nucleolar stress response
a Western-blot analysis showing the accumulation of different proteins, in 20 µg of cellular extracts produced from control U2OS cells or from U2OS cells that overexpress SURF2-Flag after Tetracycline induction (1 µg/ml) from their T-Rex locus for 24 h. Cells were also treated differentially with or without the addition of actinomycin D (10 mg/ml) for the same period (n = 3). b Quantification of the signals observed in an independent experiment (A) (n = 3). Data are presented as mean values +/− SD. One-way ANOVA tests were used for statistics. Significant differences are indicated by stars (p-value \(\le\)0.05*; \(\le\)0.01** and \(\le\)0.001***). Source data are provided as a Source Data file. c DNA content analysis of U2OS cells treated as indicated (Ctrl: U2OS control cells; OE SURF2-Flag: U2OS cells that overexpress SURF2-Flag; ACTD: Treated with actinomycin D) by FACS. Quantification of different U2OS cell repartition in the different phases of the cell cycle following different treatments is represented as histograms (n = 3). Data are presented as mean values +/− SD. One-way ANOVA tests were used for statistics. Significant differences are indicated by stars (p-value \(\le\)0.05*; \(\le\)0.01** and \(\le\)0.001***). Source data are provided as a Source Data file. d Comparison of p53 and p21 stabilization, assessed by western blots, following drugs treatment (5-Fu: 50 µM; MG-132: 10 µM; staurosporine: 25 nM for 24 h) in U2OS control cells (uninduced) or in cells overexpressing SURF2-Flag (Tetracycline 1 mg/ml). P53 and p21 signals were normalized to 1 in the control experiment, and relative stabilization in cell overexpressing SURF2-Flag was quantified from three independent biological replicates (n = 3). Data are presented as mean values +/− SD. Two-tailed paired T test was used for statistics. Significant differences are indicated by stars (p-value \(\le\)0.05*; \(\le\)0.01** and \(\le\)0.001***). Source data are provided as a Source Data file.
