Fig. 3: Hepatic SEC22B silencing affects serum and liver metabolites in fasting and refeeding conditions. | Nature Communications

Fig. 3: Hepatic SEC22B silencing affects serum and liver metabolites in fasting and refeeding conditions.

From: Phosphoproteomics-directed manipulation reveals SEC22B as a hepatocellular signaling node governing metabolic actions of glucagon

Fig. 3

a Liver triglyceride levels of male C57Bl/6 N mice administered with adeno-associated viruses expressing a microRNA to silence Sec22b (miR-Sec22b) or a negative control (miR-NC) and fasted for 16 h (fasting) or fasted and then refed for 5–6 h (refeeding). Data are mean ± s.e.m.; n = 5 mice per group. Statistical tests used were 2-way ANOVA with Holm-Sidak post-hoc tests. Dotted line shows the main effect. Difference between fasting and refeeding: #P < 0.05, ##P < 0.01, ###P < 0.001; Difference between miR-NC and miR-Sec22b: *P < 0.05, **P < 0.01, ***P < 0.001. b Liver cholesterol levels of mice as in a. c Serum triglyceride levels of mice as in a. d Serum cholesterol levels of mice as in a. e A heatmap of serum acyl-carnitine and amino acid species of mice (n = 5 mice per group except Fasting & miR-NC group: n = 4). Abbreviations for serum amino acids and acylcarnitines are provided in Supplementary Data 5. f–h Select serum acyl-carnitine species levels of mice as in e. i–k Select serum amino acid species levels of mice as in e. l–n Select serum amino acid species levels of male C57Bl/6 N mice administered with adeno-associated viruses expressing a microRNA to silence Sec22b (miR-Sec22b) or a negative control (miR-NC), with Flag-tagged Sec22b cDNA (Sec22b) or a control (Gfp), these mice are fasted and then refed for 5–6 h. Data are mean ± s.e.m.; n = 5 mice per group. Statistical tests used were 2-way ANOVA with Holm-Sidak post-hoc tests. Difference between fasting and refeeding: #P < 0.05, ##P < 0.01, ###P < 0.001; Difference between miR-NC and miR-Sec22b: *P < 0.05, **P < 0.01, ***P < 0.001. o: Liver western blot images of SEC22B, FLAG, GFP and control VCL of mice as in l. p Western blot quantification of o. Samples were obtained from the same experiment, and blots were processed simultaneously. q Liver oil-red O stain images of mice as in l. Images are representative of three individual mice per group. Scale bar: 100 µm. r Serum triglyceride levels of mice as in l. s Serum cholesterol levels of mice as in l. t Serum ALT levels of mice as in l. u Serum AST levels of mice as in l. Source data are provided as a Source Data file. Exact P-values are provided in Supplementary Data 6.

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