Fig. 3: Phylogenetic analyzes of AlaS protein sequences from bacterial and viral origin.

Unrooted maximum likelihood phylogenetic tree for AlaS. Black names denote sequences from bacteria and metagenomic contigs of uncertain origin. Bradyrhizobium sp. ORS278, containing the three gene-cassette, is marked in gold. The vAlaS sequence from CB_2 phage chosen for experimental characterization is colored pink. Metagenomically retrieved contigs from this project are color coded according to the bars in Fig. 2. Circles represent bootstrap values > 0.9. The scale bar indicates the average number of amino acid substitutions per site.