Fig. 2: Pericentral and sex-dimorphic Vldlr expression in the mouse liver. | Nature Communications

Fig. 2: Pericentral and sex-dimorphic Vldlr expression in the mouse liver.

From: A sexually dimorphic hepatic cycle of periportal VLDL generation and subsequent pericentral VLDLR-mediated re-uptake

Fig. 2

A scRNA-seq revealed a sexually dimorphic and pericentral expression of Vldlr. In females, the expression is lower at ZT22. Other lipoprotein receptors, Ldlr, Lrp1 and Lrp6, do not exhibit sexual dimorphism (n = 11; n = 2–3, per condition). B H-DAB stainings on flash frozen tissue show a pericentral confinement of VLDLR, with more intensive immunoreactivity in females. Male and female immunoreactivity is cytoplasmic at ZT10 and ZT16 while more membrane-localized at ZT22 and ZT4. Quantification of DAB staining shown as relative signal in concentric areas of 15 µm around the central vein (CV), or quantification of DAB signal at the distance of 15 µm from the central vein at different time points, errors represent SEM between biological replicates (n = 24; n = 3, per condition). C Staining of neutral lipids with BODIPY 493/503 shows a pericentral increase in neutral lipids in males at ZT10, fitting with the space-time logic of cytoplasmic VLDLR immunoreactivity. Such a phenotype is not observable in females. At ZT22 there is a lack of zonation in the BODIPY signal (n = 2–3, per condition).

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