Fig. 3: Periportal VLDL generation in the mouse liver. | Nature Communications

Fig. 3: Periportal VLDL generation in the mouse liver.

From: A sexually dimorphic hepatic cycle of periportal VLDL generation and subsequent pericentral VLDLR-mediated re-uptake

Fig. 3

A scRNA-seq revealed a periportal bias of multiple genes crucial for VLDL generation. Apoe, Apoa4, Apoc2, Apoc3 and Tm6sf2 show a periportal bias. Apob shows attenuated mRNA counts in the midlobular region (n = 11; n = 2-3, per condition). B Transmission EM images of periportal and pericentral hepatocytes. Periportal hepatocytes are rich in vesicles loaded with low density particles, whereas pericentrally these vesicles are scarce in both females and males. The scale bar represents 1 µm (n = 6 mice; n = 3, per sex). C Scanning EM allowed quantification of vesicles loaded with VLDLs in > 40000 µm3 of pericentral and periportal hepatic tissue, revealing an approximately 2-fold periportal to pericentral ratio of VLDL-loaded carriers (error bars represent SEM; n = 4 mice; p = 0.017, paired two-sided t-test).

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