Fig. 3: In vitro biocompatibility and in vitro immunomodulation of sHAChiF.
From: Sprayable anti-adhesive hydrogel for peritoneal macrophage scavenging in post-surgical applications
a Representative Live&Dead assay fluorescence images of NIH-3T3 cells incubated with sHAChiF of different compositions (Scale bar = 100 μm). b Quantification of the live NIH-3T3 cells by Live&Dead assay. c Representative iNOS and Arg1 immunofluorescence staining of LPS-treated RAW264.7 macrophages incubated with sHAChiF (Scale bar = 50 μm). d Quantification of the M1/M2 ratio of RAW264.7 macrophages. e Quantification of TNF-α secreted by LPS-treated RAW264.7 macrophages. Data are shown as mean ± SD (n = 4) independent samples in a,b; n = 4 independent samples in c,d; n = 3 independent samples in e. One-way ANOVA with Tukey post-hoc test was used for statistical analysis of b,d,e. Source data are provided as a Source Data file.
