Fig. 5: Disturbance of peritoneal macrophage aggregation by sHA in sHAChiF.

a Representative images of the aggregation behavior of rat peritoneal macrophages following treatment with various polysaccharides (Scale bar = 50 μm). b Quantification of aggregated rat peritoneal macrophages treated with various polysaccharides. c Schematic illustration of the mouse hepatic thermal injury model. d Representative images of mouse organs in the mouse hepatic thermal injury model after treatment with sHAChiF, showing F4/80 (yellow; pseudocolor), Chi (cyan; pseudocolor), and sHA (magenta; pseudocolor) (Scale bar = 10 mm). e Quantification of F4/80 expression on the wound by measuring fluorescence with APC/Fire^TM anti-F4/80 antibody, normalized by fluorescence levels in the normal liver. f Quantification fluorescence of sHA from sHA^Cy7.5 on the wound (left) and Chi from Chi^Cy5.5 on the wound (right), normalized by fluorescence from normal liver. g Schematic illustration of analysis for sHA uptake behavior of peritoneal cells using flow cytometry. Peritoneal cells gated based on sHA⁺ population (h), grouping of sHA⁺ LPM and sHA⁺ SPM by F4/80 and CD11b expression levels (i), the ratio of peritoneal cell populations distinguishing between sHA^- and sHA⁺ populations (j). Data are shown as mean ± SD (n = 3) independent samples in a,b; n = 4 independent samples in d–f; n = 4 independent samples in h–j. Two-tailed unpaired t-test was used for statistical analysis of f. One-way ANOVA with Tukey post-hoc test was used for statistical analysis of b,e. Source data are provided as a Source Data file. Image was created using PowerPoint.