Fig. 2: Expanded HTT mRNA unravels non-cooperatively.
From: Stick-slip unfolding favors self-association of expanded HTT mRNA
A Three successive cycles of stretching (black) and relaxation (red) for fHTT12-40 mRNAs, fit to eWLC models as in Fig. 1. Cooperative transitions (asterisks) become less common as the repeat expands and are lost for ∆CCG (bottom). Expected ∆Lc from the fully folded to the fully unfolded state is 36.58 nm for fHTT12; 50.74 nm for fHTT20; 86.14 nm for fHTT40; 68.44 nm for fHTT40∆CCG. B Probability density of unfolding and refolding forces for stretching (black) and relaxation (red) for each fHTT mRNA. Average unfolding force for fHTT12 was 12.4 pN, compared to 11.8 pN for fHTT7. Shaded region indicates the force range for non-cooperative stick-slip unfolding transitions.
