Fig. 5: Mapping of RNA-binding sites within RBPs.
From: Capture of RNA-binding proteins across mouse tissues using HARD-AP
a Schematic of strategies used to map RBSs using machine learning. b Sequence logos of enriched motifs of RNA-binding sites identified in globular domains of human and mouse RBPs. Number under each logo represents the number of RBPs harboring the motif. The Sequence logos were generated by MEME suite. c Bar plot showing the numbers of RBPs with indicated motifs divided into those with and without zinc finger (ZnF) domains. d Top ten GO terms in human and mouse RBPs containing RNA-binding IDRs. The GO enrichment analysis used the two-sided Fisher’s exact test with the p-value adjusted using the Bonferroni correction for multiple testing. e Sequence logos of motifs enriched in IDRs of human and mouse RBPs. Number under each logo represents the number of RBPs harboring the motif. The Sequence logos were generated by MEME suite. f Numbers of RBPs identified by HARD-AP with canonical RBDs. g Matrix bubble plot showing enrichments of previously described domains (Conserved Domain Database) in the RBPs identified using HARD-AP as well as previously described RBPs. Color scales represent the fraction of domains harboring predicted RBS. Source data for (f, g) are provided as a Source Data file.
