Fig. 5: Increased MHC-I surface expression in professional APCs upon loss of RNF185/MBRL ERAD complex. | Nature Communications

Fig. 5: Increased MHC-I surface expression in professional APCs upon loss of RNF185/MBRL ERAD complex.

From: Tapasin assembly surveillance by the RNF185/Membralin ubiquitin ligase complex regulates MHC-I surface expression

Fig. 5

a Deletion of RNF185 and MBRL in human iPSC-derived macrophages results in elevated TPSN levels. Parental, RNF185, and MBRL KO iPSCs from Fig. 1c were differentiated into macrophages. Macrophages were either left untreated or were treated for 16 h with IFN gamma (100 ng/mL). Protein extracts were analysed by SDS–PAGE and immunoblotting with the indicated antibodies. The asterisk (*) indicates a non-specific background band. b RNF185 and MBRL knockout iPSC-derived macrophages display elevated cell surface MHC-I levels as detected by flow cytometry with the conformation-sensitive W6/32 antibody. Macrophages were either left untreated or were treated for 16 h with IFN gamma (100 ng/mL). The identity of cells was confirmed with the macrophage markers CD14 and CD45.

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