Fig. 8: Analysis of mechanisms that underlie tonic cGAS-STING activation in the ISG(high) cell lines. | Nature Communications

Fig. 8: Analysis of mechanisms that underlie tonic cGAS-STING activation in the ISG(high) cell lines.

From: A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

Fig. 8

a Polyclonal populations of SCC25, H596, OE21, and Detroit 562 cells knocked out (KO) for TREX1 using two separate sgRNAs or wild-type (WT) controls were analyzed for IFIT2 expression by RT-qPCR. Data show the relative expression of IFIT2 in KO cells compared to parental controls (set to 1) from n = 3 (OE21) or 6 (SCC25, H596, Detroit 562) replicates, error bars show the SEM. b cGAS and STING variants present in the indicated cell lines derived from RNA-seq data. c, d SCC25, H596, OE21, and Detroit 562 cells grown in the presence of 10 μM VBIT-4 for 4 days (c) or 500 μM AZT for 3 days (d). IFIT1, IFIT2, and IRF7 expression was analyzed by RT-qPCR and normalized relative to mock-treated controls (set to 1). Data show the mean from n = 3 replicates, error bars show the SEM. e Following subcellular fractionation (see “Methods”), levels of the indicated HERV and LINE DNA elements were assessed by qPCR in the cytosol and nucleus. Data show the relative levels of each element in the cytosol relative to the nuclear extract in each cell type (n = 2, error bars show SEM). Source data are provided as a Source Data file.

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