Fig. 4: An SS/DS motif in Es-SSC regulates TMED10-mediated UcPS. | Nature Communications

Fig. 4: An SS/DS motif in Es-SSC regulates TMED10-mediated UcPS.

From: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors

Fig. 4

a C-terminal partial amino acid sequence alignment of indicated coronaviruses E protein. SS/DS residues of Es-SSC are marked in red. The alternative residues of Es-MSC are marked in green. b mIL1β-HA secretion in HEK293T cells transfected with Myc-tagged E WT or AA mutant of indicated severe symptom coronaviruses. c mIL1β secretion in THP-1 cells expressed with Myc-tagged E WT or AA mutant of indicated severe symptom coronaviruses. d Co-IP using HEK293T cells with TMED10-V5 and Myc-E WT or AA mutant of indicated severe symptom coronaviruses. e Cell-free membrane translocation of mIL1β-FLAG in the absence or presence of Myc-E WT or AA mutant of SARS2. f mIL1β-HA secretion in HEK293T cells transfected with Myc-tagged E-SARS2, Myc-tagged E WT or SS mutant of indicated mild symptom coronaviruses. g mIL1β secretion in THP-1 cells expressed with Myc-tagged E-SARS2, Myc-tagged E WT or SS mutant of indicated mild symptom coronaviruses. h Co-IP using HEK293T cells with TMED10-V5 and Myc-tagged E-SARS2, Myc-tagged E WT or SS mutant of indicated mild symptom coronaviruses. i–k Cell-free membrane translocation of mIL1β-FLAG in the absence or presence of Myc-tagged E-SARS2, Myc-tagged E WT or SS mutant of indicated mild symptom coronaviruses. Prot K, Protease K; TX-100, TritonX-100. Source data are provided as a Source Data file.

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