Fig. 2: Pre-stabilizing cells with formaldehyde does not impact protein labeling. | Nature Communications

Fig. 2: Pre-stabilizing cells with formaldehyde does not impact protein labeling.

From: Cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure

Fig. 2

A Average percent proteome labeling using N-(propionyloxy)succinimide across E. coli and human A549 cells with increasing concentrations of formaldehyde (n = 2 biological replicates). B Comparison of percent proteome labeling with biotin-X-NHS (blue) and sulfo-NHS-LC-biotin (orange) in A549 cells for live, fixed, and fixed + permeabilized states (n = 2 biological replicates). C Histogram of identified protein abundance from human non-labeled lysate (light orange), fixed only (purple), and D fixed + permeabilized (red), labeled with biotin-X-NHS. Protein abundancies retrieved from PaxDb46.

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