Fig. 4: Effect of fixation and permeabilization on in situ XL-MS. | Nature Communications

Fig. 4: Effect of fixation and permeabilization on in situ XL-MS.

From: Cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure

Fig. 4

A Number of Number of Crosslink Spectral Matches (CSMs, blue) and unique crosslinks (orange: loop-links, grey: intraprotein, and yellow: interprotein) identified from in situ crosslinking with DSS, in either live cells, fixed cells, fixed and permeabilized cells, or fixed cells with a 3X DSS treatment. B Number of Protein-Protein Interactions (PPIs) identified from in situ crosslinking with DSS, in either live cells, fixed cells, fixed and permeabilized cells, and fixed cells with a 3X DSS treatment. C STRING score distribution for STRING PPIs (grey), live cells (brown), fixed cells (orange), fixed + permeabilized cells (yellow), and fixed cells with a 3X DSS treatment (blue); PPIs not found in STRING database labeled as not-found (N/F).

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