Fig. 4: Assemble 100 strains.

Among these 100 strains, their average coverages follow a log-normal distribution, resulting in variations in the average coverage of each individual strain. Here, we assess the impact of different coverages on the assembly methods of HyLight, MetaPlatanus, and OPERA-MS. Different colors represent different assembly approach. (a) As coverage increases, there is a change in the genome fraction for distinct approaches. (b) As coverage increases, there is a variation in misassembly contig length among different assembly methods. c and d Increase in coverage, changes in mismatch and indel error rate in the assembly results of different approaches. Box plots represent the median (center line), the 25th and 75th percentiles (bounds of the box), and the minimum and maximum values within 1.5 times the interquartile range (whiskers).