Fig. 4: Survival analysis in advanced melanoma patients and relapse detection in adjuvant patients.

Kaplan-Meier curves and differences in survival for patients with combined ICI grouped by ctDNA AF changes between T0 and T1 for PFS (A), OS (B) and between T1 and T2 for PFS (C) and OS (p = 3.712 × 10−4) (D). AF asc—ascending or stable allele fractions over time, AF desc—descending allele fractions. E A swimmer plot with patients with adjuvant ICI and follow-up. Relapse-free patients (blue bars) had no ctDNA-positive liquid biopsy indicating a high specificity of the approach. ctDNA-negative: samples with less than three significant variants, ctDNA-positive: samples with at least three significant variants. Source data are provided as a Source Data file.