Fig. 5: AIEgen-assisted SNA reporters enhance the CrisprAIE detection of target DNA in complex samples.

a Schematic of the CrisprAIE-based SNA reporter. b Detection sensitivity of CrisprAIE-based SNA/AIEgen reporter in detecting norovirus synthetic DNA. c Calibration curve fitting the relative fluorescence intensities with target concentrations at 30 min of the detection in (b). Schematic of the stability and fluorescence kinetic analysis of ssDNA-FAM reporters (d), Q-dsDNA/AIEgen reporters (e), and SNA/AIEgen reporters (f) in 5 U mL⁻¹ DNase I or 100% FBS, respectively. g Signal-to-noise ratio (S/N) calculated by dividing the 100 pM target signals by the signal in 5 U mL⁻¹ DNase I or 100% FBS. h Comparison of detection sensitivity among all the AIEgen-enhanced reporter-based CrisprAIE and conventional FQ reporter-based Crispr-Dx in this study. S/N in the detection of 100 pM target: Norovirus ORF2 gene synthetic targets spiked into reaction buffer or 8% vomitus (i); SARS-CoV-2 N gene synthetic targets spiked into reaction buffer, throat swab or 8% saliva (j); Ebola (k), HPV-16 L1 gene (l), HPV-18 L1 gene (m), and MPXV B6R gene (n) synthetic DNA targets spiked into reaction buffer or 2.5% serum. Data are mean ± s.d.; n = 3 repeated tests. Two-tailed P values were calculated using unpaired t-tests: *P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001; ns, not significant, compared with buffer only, respectively.