Fig. 6: Droplet-based IVTT library generation and sorting using the two different droplet sorters. | Nature Communications

Fig. 6: Droplet-based IVTT library generation and sorting using the two different droplet sorters.

From: NOVAsort for error-free droplet microfluidics

Fig. 6

A Step-by-step workflow of the droplet-based IVTT assay, where the generated polydisperse library is sorted by either the linear droplet sorter or NOVAsort (scale bar = 200 µm). B Sorting results of the polydisperse IVTT droplet library (n = 1000 for the “linear sorter” group and n = 1000 for the “NOVAsort” group). “Size + ” and “Size − ” stand for droplets with correct and incorrect sizes. “Fluor + ” and “Fluor − ” stand for GFP-positive and GFP-negative droplets. “Size+ & Fluor + ” are the target hits that need to be sorted. A created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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