Fig. 1: Single-cell profiling of sncRNAs in human embryos.
From: An atlas of small non-coding RNAs in human preimplantation development
a Overview of the experimental workflow. Donated embryos were cultured from E3-E7, dissociated into single cells, and manually picked for Small-seq or Co-seq. The dotted arrow indicates where a subset of cells were used. Number of cells and embryos remaining following QC filtering were attached below (created with BioRender). b Total UMIs and (c) proportion of UMIs annotated to microRNA (miRNA), small nucleolar RNA (snoRNA), small nuclear RNA (snRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), and piwi-interacting RNA (piRNA) by day of embryonic development. The number of cells for each day is as follows: E3: 20, E4: 106, E5: 304, E6: 394, E7: 148. The boxplot rectangles represent the first and third quartiles, a horizontal line inside the box indicates the median value. d Percentage of total annotated reads by sncRNA class of various sequence lengths. e Gene expression of sncRNA processing genes across embryonic development and lineage (inner cell mass (ICM) and trophectoderm (TE)). Error bars represent standard error of the mean (SEM) values. The number of cells for each day is as follows: E3: 61, E4: 144, E5: 245, E6: 278, E7: 361. Created in BioRender. Kwok, Y. (2023) BioRender.com/v41c600.
