Fig. 2: PDCD2 interacts with AtPRMT3 and RPS2B, and mutation of PDCD2 partially rescues the defects of rps2a2b mutants.
From: AtPRMT3-RPS2B promotes ribosome biogenesis and coordinates growth and cold adaptation trade-off
a Protein schematic structures of PDCD2 and its ΔZF version. b, c Phenotypes of ten-day-old seedlings of Col-0, atprmt3-2, and PDCD2E350K&ΔZF-HA are shown. Scale bar, 1 cm. Data are presented as mean values ± SD (n = 25). d, e The interaction between PDCD2 and RPS2B validated by yeast two-hybrid assay (d) and luciferase complementation assay (e). f The interaction between PDCD2 and AtPRMT3 validated by luciferase complementation assay. g, h Phenotypes of ten-day-old seedlings of Col-0, rps2a2b-1, and rps2a2bpdcd2-d1 are shown. Scale bars, 1 cm. Data are presented as mean values ± SD (n = 25). i Pre-rRNA processing states. Northern blot was performed with probe S7, methylene blue (MB) stain was used as a loading control. All experiments were repeated at least three times with similar results. P value was calculated by One-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.
