Fig. 4: The atprmt3 mutants exhibit freezing tolerant phenotype and rescued by mutation of PDCD2.
From: AtPRMT3-RPS2B promotes ribosome biogenesis and coordinates growth and cold adaptation trade-off
a Polysome profiles of Col-0, atprmt3-2, and atprmt3pdcd2-d1. The x-axis indicates 5–50% sucrose gradient, y-axis indicates OD254. b Statistical analysis for relative OD254 signal ratios of polysome/total in (a). Data are presented as mean values ± SD (n = 3). c Volcano plots of differentially expressed genes in atprmt3-2 and atprmt3pdcd2-d1 compared with Col-0. d Gene ontology (Biological process) of the up-regulated genes that are rescued in suppressor atprmt3pdcd2-d1. Font size correlates to -Log10FDR. e The expression pattern of AtPRMT3 and PDCD2 in shoot under various abiotic stress conditions. Data are presented as mean values ± SD. f The expression of AtPRMT3 and PDCD2 in response to cold stress. Total RNA from twelve-day-old Col-0 seedlings treated at 4 °C for 0, 12, and 24 h were used for qRT-PCR. UBQ10 was used as internal control. Data are presented as mean values ± SD (n = 4). g Freezing stress phenotypes. Twelve-day-old seedlings of Col-0, atprmt3-2, atprmt3pdcd2-d1, and complementation lines PDCD2E350K-HA were subjected to freezing treatment at −6 °C for 1 h for non-acclimated (NA) condition or −8 °C for 3 h for cold-acclimated (CA) condition. h The survival rates of plants in (g). Data are presented as mean values ± SD (n = 4). P value was calculated by One-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.
