Fig. 5: AtPRMT3-RPS2 co-regulate transcriptome and translatome changes.
From: AtPRMT3-RPS2B promotes ribosome biogenesis and coordinates growth and cold adaptation trade-off
a Freezing stress phenotype. Twelve-day-old seedlings of Col-0, atprmt3-2, rps2a2b-1, and rps2c-1 were subjected to freezing treatment at −6 °C for 1 h under NA condition. b The survival rates of plants in (a). Data are presented as mean values ± SD (n = 4). c Polysome profiles of Col-0, atprmt3-2, rps2a2b-1, and rps2c-1 mutants. The x-axis indicates 5–50% sucrose gradient, y-axis indicates OD254. d Statistical analysis for relative OD254 signal ratios of polysome/total in (c). Data are presented as mean values ± SD (n = 3). e Schematic representation of RNA-seq and Ribo-seq analysis. f The correlation between translational and transcriptional changes. Scatter plots comparing fold-changes (FC) of FPKM between RNA-seq (x-axis) and Ribo-seq (y-axis) are shown. Pearson r and two-tailed p value are shown. (b, d) P value was calculated by One-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.
